Journal: Journal of Molecular Histology
Article Title: Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways
doi: 10.1007/s10735-025-10423-5
Figure Lengend Snippet: CENPM promoted NSCLC development through Wnt/β-catenin signaling a Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis in CENPM high ( n = 126, relative CENPM expression > 100) in comparison with low CENPM ( n = 85, relative CENPM expression < 100) NSCLC patients. b Cell proliferation of vector A549/NCI-H1299, CENPM overexpression A549/NCI-H1299 treated with PBS, PKI (20 nM), PNU (10 nM) and KAAD (10 nM) respectively. c Colony formation of vector A549/NCI-H1299, CENPM overexpression A549/NCI-H1299 treated with PBS, PKI (20 nM), PNU (10 nM) and KAAD (10 nM) respectively. d Western blotting of Wnt-3a and β-catenin in vector and CENPM overexpression A549/NCI-H1299. e Immunofluorescence of Wnt-3a in NSCLC tissues divided into metastatic (M1, n = 13) and non-metastatic (M0, n = 13) groups. The scale bar was 50 μm. Expression intensity were quantified in 26 patients
Article Snippet: Cancer cells were maintained in Roswell Park Memorial Institute (RPMI)-1640 medium (Thermo Fisher, USA) containing 10% fetal calf serum (FBS) in a standard atmosphere with 5% CO 2 , and at a temperature of 37 °C. cAMP inhibitor PKI 14–22 amide, myristoylated (PKI), Wnt/β-catenin inhibitor PNU-74,654 (PUN), hedgehog signaling inhibitor KAAD-Cyclopamine (KAAD) were purchased from Medchemexpress (USA).
Techniques: Expressing, Comparison, Plasmid Preparation, Over Expression, Western Blot, Immunofluorescence